c57bl 6 mouse complement serum Search Results


mouse serum complement  (Innovative Research Inc)
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Innovative Research Inc mouse serum complement
Figure 1. Preparation of the positive control. The positive control was used as quality control in the enzyme-linked immunosorbent assay (ELISA). Mouse serum con- taining mouse immunoglobulin (Ig) and inactivated <t>complement</t> was incubated with heat-aggregated human IgG (“Complement Activator”) to stimulate complement activation by the classical complement pathway. Monomeric mouse IgG was able to bind to aggregated human IgG, leading to formation of complement bound hIgG-mIgG complexes. HRP (horse radish peroxidase) signal.
Mouse Serum Complement, supplied by Innovative Research Inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Figure 1. Preparation of the positive control. The positive control was used as quality control in the enzyme-linked immunosorbent assay (ELISA). Mouse serum con- taining mouse immunoglobulin (Ig) and inactivated complement was incubated with heat-aggregated human IgG (“Complement Activator”) to stimulate complement activation by the classical complement pathway. Monomeric mouse IgG was able to bind to aggregated human IgG, leading to formation of complement bound hIgG-mIgG complexes. HRP (horse radish peroxidase) signal.

Journal: Journal of Immunotoxicology

Article Title: An ELISA for detection of complement-bound circulating immune complexes in mice

doi: 10.1080/1547691x.2019.1599471

Figure Lengend Snippet: Figure 1. Preparation of the positive control. The positive control was used as quality control in the enzyme-linked immunosorbent assay (ELISA). Mouse serum con- taining mouse immunoglobulin (Ig) and inactivated complement was incubated with heat-aggregated human IgG (“Complement Activator”) to stimulate complement activation by the classical complement pathway. Monomeric mouse IgG was able to bind to aggregated human IgG, leading to formation of complement bound hIgG-mIgG complexes. HRP (horse radish peroxidase) signal.

Article Snippet: In brief, mouse serum complement (Innovative Research, Novi, MI, cat. no IMS-C57BL6-COMPL) was incubated at 37 C for 90min with 1:100 HAGG (Complement Activator, Quidel, San Diego, CA, cat. #A114), followed by inactivation by addition of ice-cold 500mM ethylenediamine-tetraacetic acid (EDTA; to final concentration of 20mM) and storage (undiluted) at 80 C until analysis.

Techniques: Positive Control, Control, Enzyme-linked Immunosorbent Assay, Incubation, Activation Assay